Human CYP24A1 (Cytochrome P450, Family 24, Subfamily A, Poly Peptide 1) ELISA Kit
| Target Information | |
|---|---|
| Target Name | CYP24A1 (Cytochrome P450, Family 24, Subfamily A, Poly Peptide 1) |
| UniProt ID | Q07973 |
| Synonyms | CP24, CYP24, HCAI, P450-CC24, 1,25-dihydroxyvitamin D3 24-hydroxylase, mitochondrial |
| Product Properties | |
|---|---|
| Prize | Online Inquiry |
| Reactivity | Human |
| Assay Type | Sandwich-ELISA |
| Assay Time | 3.5 h |
| Detection Range | 0.16-10 ng/mL |
| Sample Type | Serum, plasma and other biological fluids |
| Sample Volume | 100 μL |
| Sensitivity | 0.10 ng/mL |
| Specificity | This kit recognizes Human CYP24A1 in samples. No significant cross-reactivity or interference between Human CYP24A1 and analogues was observed. |
| Research Area | Cancer, Cardiovascular, Metabolism, Signal Transduction |
| Reproducibility | Both intra-CV and inter-CV are < 10%. |
| Precision | Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level human CYP24A1 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level human CYP24A1 were tested on 3 different plates, 20 replicates in each plate. |
| Storage | -20 ℃. Please refer to the instructions for details. |
Application
This ELISA kit applies to the in vitro quantitative determination of Human CYP24A1 concentrations in serum, plasma and other biological fluids.
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to human CYP24A1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for human CYP24A1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain human CYP24A1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of human CYP24A1. You can calculate the concentration of human CYP24A1 in the samples by comparing the OD of the samples to the standard curve.
! For research use only. Not intended for any clinical use.