Custom Animal Models of Thrombosis
Inquiry
The global incidence of thrombotic events is increasing every year. Among them, arteriovenous thrombosis is the key pathogenic mechanism of ischemic heart disease and stroke. Due to the widespread and critical role of thrombosis in many cardiovascular diseases, Ace Therapeutics has developed various animal models of thrombosis to help our clients explore the pathophysiology and development of novel therapeutic agents.
Thrombosis Related Diseases
Thrombosis, the formation of intravascular clots, can have serious consequences, including arterial embolism and venous embolism, and it is the underlying cause of the two most common cardiovascular diseases, acute myocardial infarction and cerebrovascular accidents. Considering the widespread and critical role of thrombosis in many diseases, animal models of thrombosis are needed to explore its pathophysiology and to develop safer and more effective drugs.
Fig. 1 Triggers of arterial and venous thrombosis. (Mackman N, 2008)
Our Services
Ace Therapeutics can offer a variety of development services for animal models of thrombosis, including but not limited to:
Inferior Vena Cava Ligation
Inferior Vena Cava Stenosis
Mechanical Injury
Pro-coagulant Intervention
Ferric Chloride Induced
Animal Models of Thrombosis Induced by Inferior Vena Cava Ligation
- Overview
We can perform surgical ligation of the inferior vena cava in an animal model, which leads to stagnation of blood flow, further ischemic and hypoxic damage to the venous endothelial cells, stimulating the production and release of coagulation factors and promoting the formation of intravascular thrombi.
- Available Animal Species and Strains
Rats (SD rats, Wistar rats), rabbits (New Zealand white rabbit, Japanese white rabbit)
Animal Models of Thrombosis Induced by Inferior Vena Cava Stenosis
- Overview
Distinct from the inferior vena cava ligation method, we can perform a partial ligation procedure on the inferior vena cava in an animal model to narrow the lumen of the inferior vena cava and ultimately form a thrombus at the site of the narrowed vein.
- Available Animal Species and Strains
Rats (SD rats, Wistar rats), rabbits (New Zealand white rabbit, Japanese white rabbit)
Animal Models of Thrombosis Induced by Mechanical Injury
- Overview
For some large experimental animals, we can use the mechanical injury method, using surgical instruments to artificially cause damage to the endothelium of the veins of the experimental animals, thus activating the coagulation mechanism of the experimental animals and promoting the formation of deep vein thrombosis.
- Available Animal Species and Strains
Rats (SD rats, Wistar rats), rabbits (New Zealand white rabbit, Japanese white rabbit)
Animal Models of Thrombosis Induced by Pro-coagulant Intervention
- Overview
We can induce deep vein thrombosis in a non-surgical and traumatic manner by injecting procoagulants into the venous vasculature of the animal and directly activating coagulation factors in the blood vessels.
- Available Animal Species and Strains
Mice (C57BL/6 mice, BALB/c mice), rats (SD rats, Wistar rats), rabbits (New Zealand white rabbit, Japanese white rabbit)
Animal Models of Thrombosis Induced by Ferric Chloride (FeCl3)
- Overview
The ferric chloride method is a well-researched method for inducing thrombosis in animal models. After the experimental animals are anesthetized, we surgically isolate and expose the inferior vena cava of the abdomen, and apply ferric chloride solution externally to the blood vessels, thereby inducing vascular lipid peroxidation and endothelial cell destruction, activating the coagulation cascade reaction, and ultimately leading to the formation of blood clots.
- Available Animal Species and Strains
Mice (C57BL/6 mice, BALB/c mice), rats (SD rats, Wistar rats), rabbits (New Zealand white rabbit, Japanese white rabbit)
Animal Model Validation
For the animal model of deep vein thrombosis, we can choose to test the coagulation indexes (PT, APTT, TT, FIB, FDP, D-Dimer, AT-III), ultrasound imaging to evaluate the length and size of thrombus, and HE staining of thrombus pathological sections to comprehensively evaluate whether the construction of the animal model of deep vein thrombosis is successful or not.
Ace Therapeutics has accumulated extensive experience in the construction and application of experimental animal models. Arterial and venous thrombosis is the most important causal mechanism of ischemic heart disease and stroke, and we can develop relevant animal models of thrombosis according to the customized needs of our clients, so as to support the basic research and drug development of various thrombosis-related diseases. If you are interested in our services, please don't hesitate to contact us.
Reference
- Mackman, N. Triggers, targets, and treatments for thrombosis. Nature. 2008, 451(7181): 914-918.
! All of our services and products are intended for preclinical research use only and cannot be intended for any clinical use.
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