Custom Animal Models of Heart Failure
Inquiry
Heart failure is a complex clinical syndrome induced by a variety of factors, and its causes and mechanisms of action are complex. Heart failure is a pathophysiological state and clinical syndrome in which the heart's pumping function is reduced, and the blood returned from the veins cannot be discharged sufficiently to meet the metabolic needs of the body's tissues. Ace Therapeutics has always been committed to the development of cardiovascular disease models, which can provide customers with various types of animal models of heart failure.
Heart Failure Models Induced by Different Methods
There are many different causes of heart failure, and a variety of diseases can lead to a weakening of the contractility of the heart muscle, which reduces the amount of blood expelled from the heart to meet the needs of the body, and results in a series of signs and symptoms. Because of this, animal models of heart failure have been established in a variety of ways, and the changes in cardiac function and characteristics of various models are different. The main modeling methods include drug-induced heart failure, high-fat diet-induced heart failure, and surgical narrowing of blood vessels to trigger heart failure.
Fig. 1 Graphical summary of large animal models of HFrEF. (Spannbauer A, et al., 2019)
Our Services
Ace Therapeutics offers the following development services for animal models of heart failure, including but not limited to:
Essential HF Models
Diet induced HF Models
Drug Induced HF Models
Surgery Induced HF Models
Essential Heart Failure Animal Models
- Overview
Heart failure can be caused by a variety of basic diseases. We can provide customers with animal models of primary heart failure caused by hypertension, myocardial hypertrophy, obesity, and aging, which are suitable for use in basic research on the pathogenesis of heart failure.
- Available Animal Species and Strains
Spontaneously aging-prone (SAMP8) mice, spontaneously hypertensive (SHR) rats, SHHF/Mcc-/facp rats
- Animal Model Validation
Due to the long formation time of the animal model of essential heart failure, we will conduct blood biomarker analysis (ANP, BNP, cTn, Gal-3), electrocardiography, echocardiography, and myocardial pathology biopsy analysis (HE staining, Masson staining, and TUNEL staining) to analyze and validate whether the experimental animals developed heart failure or not.
Animal Models of Heart Failure Induced by High-Fat Diets
- Overview
By feeding experimental animals a high-fat diet for a long period of time, we can induce hyperlipidemia, atherosclerosis and plaque in experimental animals, which narrow the cardiovascular wall and obstruct blood flow, leading to cardiac ischemia and ultimately heart failure. This approach is able to simulate an animal model of heart failure induced by obesity and is suitable for research work related to obesity-induced heart failure.
- Available Animal Species and Strains
Mice (C57BL/6 mice, BALB/c mice), rats (SD rats, Wistar rats)
- Animal Model Validation
Since this method simulates the pathological process of heart failure naturally triggered by obesity, it leads to the inability to accurately predict modeling time. Therefore, we will analyze and verify the success of heart failure modeling by performing blood biomarker analysis as well as electrocardiogram and echocardiogram tests, and myocardial pathology biopsy on experimental animals at regular intervals from 3-12 months after high-fat rearing.
Animal Models of Heart Failure Induced by Drugs
- Overview
We induce heart failure in experimental animals by injecting compounds (such as doxorubicin, Isoprenaline, sodium pentobarbital and ethanol) that cause abnormal myocardial function or cardiac damage, leading to inhibition of myocardial contractile function or cardiac damage. Drug-induced heart failure models are easy to establish and reproducible, and are suitable for studies that require a large number of animal models.
- Available Animal Species and Strains
Mice (C57BL/6 mice, BALB/c mice), rats (SD rats, Wistar rats)
- Animal Model Validation
After the experimental animals are administered the drug and raised normally for 2 weeks, we will perform electrocardiogram and echocardiogram tests as well as TTC tests on the experimental animal model to determine whether the heart failure model is successfully constructed.
Animal Models of Heart Failure Induced by Surgery
- Overview
We can reduce the caliber of the aorta or coronary vessels by using wire ligatures or special arterial clips, which in turn leads to obstruction of the ventricular blood outflow tracts, increasing the cardiac pressure load in the experimental animals and inducing the generation of animal models of heart failure.
- Available Animal Species and Strains
Mice (C57BL/6 mice, BALB/c mice), rats (SD rats, Wistar rats)
- Animal Model Validation
For the acute heart failure models, we will perform ECG test, cardiac ultrasound test, and TTC test on the animal model at 4 hours after surgery to verify whether the acute heart failure model was successfully induced. For the chronic heart failure models, we will perform ECG test, cardiac ultrasound test, and TTC test on the animal model at 5-7 days after surgery to verify whether the chronic heart failure model was successfully constructed.
Ace Therapeutics has been dedicated to the field of cardiovascular disease for many years and has rich experience in the construction of animal models of cardiovascular disease. We are able to design custom animal models of heart failure according to the client's individual experimental needs, which can support the study of pathogenesis, the discovery of novel biomarkers, and the development of novel therapeutic approaches related to heart failure. If you are interested in our services, please don't hesitate to contact us.
Reference
- Spannbauer, A.; et al. Large animal models of heart failure with reduced ejection fraction (HFrEF). Front Cardiovasc Med. 2019, 6:117.
! All of our services and products are intended for preclinical research use only and cannot be intended for any clinical use.
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