Custom Animal Models of Cardiac Ischemia-Reperfusion

Myocardial ischemia-reperfusion injury model is a common model for simulating human myocardial infarction and reperfusion therapy and evaluating the efficacy of anti-myocardial ischemic drugs. Ace Therapeutics offers animal model development services for ischemia-reperfusion, which can facilitate basic research and drug development in cardiovascular disease.

Overview of Cardiac Ischemia-Reperfusion

Myocardial infarction (MI) and heart failure are among the leading causes of death in the global population. Myocardial ischemia-reperfusion-induced arrhythmia is a common experimental animal model for the study of ischemic cardiomyopathy. Myocardial ischemia-reperfusion injury refers to the interruption of myocardial ischemia for a short period of time and the restoration of blood supply within a certain period. However, myocardial injury during myocardial ischemia/reperfusion (MI/R) is inevitable, causing a series of damaging changes such as calcium overload, increased oxygen free radicals (OFR), impaired myocardial fiber energy metabolism, inflammatory response, and myocardial cell apoptosis. Therefore, it is also important research work to study how to reduce myocardial injury during reperfusion.

Fig. 1 The signaling pathways involved in myocardial IPC and RPCT. (Jiang W, et al., 2022)

Ace Therapeutics aims to provide development services for animal models of cardiac ischemia-reperfusion, supporting our clients in basic research and therapeutic development for ischemic cardiomyopathy, including but not limited to:

Animal Models of Ischemia-Reperfusion Induced by Surgical Ligation of Coronary Arteries

• Overview
  At present, surgical induction is still the most important way to construct myocardial ischemia-reperfusion animal models. We can provide a variety of experimental animal ischemia-reperfusion model construction services. Specifically, we can be under deep anesthesia, surgically open the chest cavity of the animal, briefly ligate the anterior descending branch of the left coronary artery, reach the time of ischemia, untie the ligature of the live knot, close the thoracic cavity, and suture the muscle skin. After completing the surgical modeling, we will continue to monitor the animal model's activity and feeding to prevent the animal from dying of infection and other conditions.
• Available Animal Species and Strains
  Mice (C57BL/6 mice, BALB/c mice), rats (SD rats, Wistar rats), rabbits (New Zealand white rabbit, Japanese white rabbit)
• Animal Model Validation
  The ECG of the animal model was monitored at all times during the procedure, and a significant elevation of the ST2 segment in the ECG could be seen if coronary artery ligation was successful. After surgery, myocardial tissue sampling and TTC-Evans blue staining are performed for infarct area calculation.

Ace Therapeutics has been committed to the development of experimental animal models for a long time. At present, we have the platform to develop animal models of myocardial ischemia-reperfusion, which can provide customers with animal models of myocardial ischemia-reperfusion for basic research on myocardial ischemia and drug evaluation experiments. If you are interested in our services, please don't hesitate to contact us.

1. Jiang, W.; et al. Opportunities and challenges of pain-related myocardial ischemia-reperfusion injury. Front Physiol. 2022, 13:900664.