8-epi-PGF2α (8-epi-prostaglandin F2 Alpha) ELISA Kit
| Target Information | |
|---|---|
| Target Name | 8-epi-PGF2α (8-epi-prostaglandin F2 Alpha) |
| Synonyms | 15-F2t-IsoP, 8-iso-PGF2a, 8-isoprostane, 8-iso-PG, 8-iso Prostaglandin |
| Product Properties | |
|---|---|
| Reactivity | Universal |
| Assay Type | Competitive-ELISA |
| Assay Time | 2.0 h |
| Detection Range | 15.63-1000 pg/mL |
| Sample Type | Serum, plasma and other biological fluids |
| Sample Volume | 50 μL |
| Sensitivity | 9.38 pg/mL |
| Specificity | This kit recognizes 8-epi-PGF2α in samples. No significant cross-reactivity or interference between 8-epi-PGF2α and analogues was observed. |
| Research Area | Cardiovascular, Signal Transduction |
| Reproducibility | Both intra-CV and inter-CV are < 10%. |
| Precision | Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level 8-epi-PGF2α were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level 8-epi-PGF2α were tested on 3 different plates, 20 replicates in each plate. |
| Storage | -20 ℃. Please refer to the instructions for details. |
Application
This ELISA kit applies to the in vitro quantitative determination of 8-epi-PGF2α concentrations in serum, plasma and other biological fluids.
Test Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 8-epi-PGF2α. During the reaction, 8-epi-PGF2α in the sample or standard competes with a fixed amount of 8-epi-PGF2α on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 8-epi-PGF2α. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 8-epi-PGF2α in tested samples can be calculated by comparing the OD of the samples to the standard curve.
! For research use only. Not intended for any clinical use.